abstract：:Several reports of promoters from plants, viral and artificial origin that confer high constitutive expression are known. Among these the CaMV 35S promoter is used extensively for transgene expression in plants. We identified candidate promoters from Arabidopsis based on their transcript levels (meta-analysis of avail...

journal_title：Transgenic research

authors： Agarwal P,Garg V,Gautam T,Pillai B,Kanoria S,Burma PK

更新日期：2014-04-01 00:00:00

abstract：:Phytocystatins are proteinaceous inhibitors of cysteine proteases. They have been implicated in the regulation of plant protein turnover and in defense against pathogens and insects. Here, we have characterized an Arabidopsis phytocystatin family gene, Arabidopsis thaliana phytocystatin 4 (AtCYS4). AtCYS4 was induced ...

journal_title：Transgenic research

authors： Je J,Song C,Hwang JE,Chung WS,Lim CO

更新日期：2014-02-01 00:00:00

abstract：:Polyamines, spermidine, spermine and their precursor putrescine, are ubiquitous cell components essential for normal cell growth. Increased polyamine levels and enhanced biosynthesis have been associated with malignant transformation and tumor formation, and thus, the polyamines have been considered to be a meaningful...

journal_title：Transgenic research

authors： Pietilä M,Dhungana H,Uimari A,Sironen R,Alhonen L

更新日期：2014-02-01 00:00:00

abstract：:Camelina (Camelina sativa (L.) Crantz) is a re-emergent oilseed crop that is also becoming important as a model for applied projects based on studies in Arabidopsis thaliana, since the two species are closely related members of the tribe Camelineae of the Brassicaeae. Since camelina can be transformed genetically by f...

journal_title：Transgenic research

authors： Julié-Galau S,Bellec Y,Faure JD,Tepfer M

更新日期：2014-02-01 00:00:00

abstract：:The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of sev...

journal_title：Transgenic research

authors： de Paiva Rolla AA,de Fátima Corrêa Carvalho J,Fuganti-Pagliarini R,Engels C,do Rio A,Marin SR,de Oliveira MC,Beneventi MA,Marcelino-Guimarães FC,Farias JR,Neumaier N,Nakashima K,Yamaguchi-Shinozaki K,Nepomuceno AL

更新日期：2014-02-01 00:00:00

abstract：:We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked...

journal_title：Transgenic research

authors： Hisatsune C,Ogawa N,Mikoshiba K

更新日期：2013-12-01 00:00:00

abstract：:Powdery mildew (PM) is one of the major plant pathogens. The conventional method of PM control includes frequent use of sulfur-based fungicides adding to production costs and potential harm to the environment. PM remains a major scourge for Rosaceae crops where breeding approaches mainly resort to gene-for-gene resist...

journal_title：Transgenic research

authors： Jiwan D,Roalson EH,Main D,Dhingra A

更新日期：2013-12-01 00:00:00

abstract：:This study was performed to qualify goat fetal fibroblast (GFF) cell lines for genetic modification and somatic cell nuclear transfer (SCNT) to produce human lysozyme (hLYZ) transgenic goats. Nine GFF cell lines were established from different fetuses, and the proliferative lifespan and chromosomal stability were anal...

journal_title：Transgenic research

authors： Liu J,Luo Y,Zheng L,Liu Q,Yang Z,Wang Y,Su J,Quan F,Zhang Y

更新日期：2013-10-01 00:00:00

abstract：:Myostatin is a well-known negative regulator of skeletal muscle growth. Inhibition of myostatin activity results in increased muscle mass. Myostatin propeptide, as a myostatin antagonist, could be applied to promote meat production in livestock such as pigs. In this study, we generated a transgenic mouse model express...

journal_title：Transgenic research

authors： Wang K,Li Z,Li Y,Zeng J,He C,Yang J,Liu D,Wu Z

更新日期：2013-10-01 00:00:00

abstract：:The activated cannabinoid receptor 1 (CB1R) is exclusively responsible for food intake and weight gain and regulates several pathological features associated with obesity in mammals. However, the precise role of CB1R in non-mammalian model systems is poorly understood. To investigate the functions of CB1R in zebrafish...

journal_title：Transgenic research

authors： Pai WY,Hsu CC,Lai CY,Chang TZ,Tsai YL,Her GM

更新日期：2013-08-01 00:00:00

abstract：:Autosomal dominant polycystic kidney disease (ADPKD) is a common human genetic disease, affecting millions of people worldwide. The progressive growth of cysts in kidneys eventually leads to renal failure in 50 % of patients, and there is currently no effective treatment. Various murine models have been studied to elu...

journal_title：Transgenic research

authors： He J,Ye J,Li Q,Feng Y,Bai X,Chen X,Wu C,Yu Z,Zhao Y,Hu X,Li N

更新日期：2013-08-01 00:00:00

abstract：:Stable transgenic germlines carrying the red fluorescence protein (RFP) gene (rfp) driven by fast skeletal myosin light chain-2 gene (mlc2f) promoter were established in a truly euryhaline fish species, the marine medaka (Oryzias dancena; Beloniformes). Transgenic lines contained transgene copy numbers varying from a ...

journal_title：Transgenic research

authors： Cho YS,Lee SY,Kim DS,Nam YK

更新日期：2013-08-01 00:00:00

abstract：:Hematopoietic stem cells (HSC) are found in several independent sites embryonically. Loss-of-function studies indicated that Notch1, but not Notch2 signaling was required for HSC emergence from the aortic-gonado-mesonephros (AGM) region. We previously showed that constitutive Notch1 activation impaired primitive eryth...

journal_title：Transgenic research

authors： Tang Y,Bai H,Urs S,Wang Z,Liaw L

更新日期：2013-04-01 00:00:00

abstract：:The NOD.Cg-Prkdc ( scid ) Il2rg ( tm1Wjl )/SzJ mouse strain, commonly known as NSG (for NOD SCID Gamma) is severely immunodeficient and thus is an excellent recipient for xenografts, and in particular fo...

journal_title：Transgenic research

authors： Landel CP,Dunlap J,Patton JB,Manser T

更新日期：2013-02-01 00:00:00

abstract：:To identify ways to improve the efficiency of generating chimeric mice via microinjection of blastocysts with ES cells, we compared production and performance of ES-cell derived chimeric mice using blastocysts from two closely related and commonly used sub-strains of C57BL/6. Chimeras were produced by injection of the...

journal_title：Transgenic research

authors： Fielder TJ,Yi CS,Masumi J,Waymire KG,Chen HW,Wang S,Shi KX,Wallace DC,MacGregor GR

更新日期：2012-12-01 00:00:00

abstract：:Watermelon, an important fruit crop worldwide, is prone to attack by several viruses that often results in destructive yield loss. To develop a transgenic watermelon resistant to multiple virus infection, a single chimeric transgene comprising a silencer DNA from the partial N gene of Watermelon silver mottle virus (W...

journal_title：Transgenic research

authors： Lin CY,Ku HM,Chiang YH,Ho HY,Yu TA,Jan FJ

更新日期：2012-10-01 00:00:00

abstract：:Both drought and high salinity stresses are major abiotic factors that limit the yield of agricultural crops. Transgenic techniques have been regarded as effective ways to improve crops in their tolerance to these abiotic stresses. Functional characterization of genes is the prerequisite to identify candidates for suc...

journal_title：Transgenic research

authors： Jiang SY,Bhalla R,Ramamoorthy R,Luan HF,Venkatesh PN,Cai M,Ramachandran S

更新日期：2012-08-01 00:00:00

abstract：:Vesicular stomatitis virus glycoprotein (VSVG) pseudotyped murine leukemia virus (MLV) virions can transduce schistosomes, leading to chromosomal integration of reporter transgenes. To develop VSVG-MLV for functional genomics in schistosomes, the influence of the chicken β-globin cHS4 element, a prototypic chromatin i...

journal_title：Transgenic research

authors： Suttiprapa S,Rinaldi G,Brindley PJ

更新日期：2012-06-01 00:00:00

abstract：:In this study, we compared the gene targeting efficiencies of two rAAV-BRCA1 KO targeting constructs in Yucatan and Göttingen minipig fibroblasts. The homology arms of the constructs consisted exclusively of exonic sequences amplified by PCR from Yucatan genomic DNA. The sequences were identical to those of the refere...

journal_title：Transgenic research

authors： Luo Y,Bolund L,Sørensen CB

更新日期：2012-06-01 00:00:00

abstract：:We describe a transgenic microalgal Δ9-elongase pathway transformed in both Brassica napus and Arabidopsis thaliana seed resulting in the production of arachidonic acid (ARA). This pathway is noteworthy for both the production of ARA in seed tissue and the low levels of intermediate C20 fatty acids that accumulate. We...

journal_title：Transgenic research

authors： Petrie JR,Shrestha P,Belide S,Mansour MP,Liu Q,Horne J,Nichols PD,Singh SP

更新日期：2012-02-01 00:00:00

abstract：:In addition to its well-recognized antimicrobial properties, lysozyme can also modulate the inflammatory response. This ability may be particularly important in the gastrointestinal tract where inappropriate inflammatory reactions can damage the intestinal epithelium, leading to significant health problems. The consum...

journal_title：Transgenic research

authors： Cooper CA,Brundige DR,Reh WA,Maga EA,Murray JD

更新日期：2011-12-01 00:00:00

abstract：:The E8 promoter, a tomato fruit-ripening-specific promoter, and the CaMV 35S promoter, a constitutive promoter, were used to express the miraculin gene encoding the taste-modifying protein in tomato. The accumulation of miraculin protein and mRNA was compared among transgenic tomatoes expressing the miraculin gene dri...

journal_title：Transgenic research

authors： Hirai T,Kim YW,Kato K,Hiwasa-Tanase K,Ezura H

更新日期：2011-12-01 00:00:00

abstract：:Hepatitis C virus (HCV) infection is a leading cause of chronic liver disease worldwide. Since several aspects of the infection remain unresolved, there is a pressing need for a convenient animal model that can mimic the clinical disease and aid the evaluation of treatment strategies. Although some success has been ac...

journal_title：Transgenic research

authors： Desai MM,Tumurbataar B,Zhang Y,Chan LN,Sun J,Chan TS

更新日期：2011-12-01 00:00:00

abstract：:The development of animal biotechnology has led to an increase in attention to biosafety issues. Here we evaluated the impact of genetically engineered cows on the environment. The probability of horizontal gene transfer and the impact on the microbial communities in cow gut and soil were tested using three varieties ...

journal_title：Transgenic research

authors： Xu J,Zhao J,Wang J,Zhao Y,Zhang L,Chu M,Li N

更新日期：2011-10-01 00:00:00

abstract：:Although the crustacean Artemia has been commonly used as an experimental organism and served as a live bait feed for aquaculture, gene transfer system on Artemia sp. to generate stable lines is not well developed. In this study, we optimized a condition for cyst-eletroporation and generated stable lines of transgenic...

journal_title：Transgenic research

authors： Chang SH,Lee BC,Chen YD,Lee YC,Tsai HJ

更新日期：2011-10-01 00:00:00

abstract：:The seed-based production of recombinant proteins is an efficient strategy to achieve the accumulation, correct folding, and increased stability of these recombinant proteins. Among potential plant molecular farming systems, soybean [Glycine max (L.) Merrill] is a viable option for the production of recombinant protei...

journal_title：Transgenic research

authors： Cunha NB,Murad AM,Ramos GL,Maranhão AQ,Brígido MM,Araújo AC,Lacorte C,Aragão FJ,Covas DT,Fontes AM,Souza GH,Vianna GR,Rech EL

更新日期：2011-08-01 00:00:00

abstract：:Carbonic anhydrase VI (CAVI) is the only secreted isozyme of the α-carbonic anhydrase family, which catalyzes the reversible reaction [Formula in text]. It appears that CAVI protects teeth and gastrointestinal mucosa by neutralizing excess acidity. However, the evidence for this physiological function is limited, and ...

journal_title：Transgenic research

authors： Pan PW,Käyrä K,Leinonen J,Nissinen M,Parkkila S,Rajaniemi H

更新日期：2011-06-01 00:00:00

abstract：:Mice from the inbred C57BL/6 strain have been commonly used for the generation and analysis of transgenic and knockout animal models. However, several C57BL/6 substrains exist, and these are genetically and phenotypically different. In addition, each of these substrains can be purchased from different animal providers...

journal_title：Transgenic research

authors： Zurita E,Chagoyen M,Cantero M,Alonso R,González-Neira A,López-Jiménez A,López-Moreno JA,Landel CP,Benítez J,Pazos F,Montoliu L

更新日期：2011-06-01 00:00:00

abstract：:Mammalian Heat Shock Proteins (HSP), have potent immune-stimulatory properties due to the natural capability to associate with polypeptides and bind receptors on antigen presenting cells. The present study was aimed to explore whether plant HSP, and in particular HSP70, share similar properties. We wanted in particula...

journal_title：Transgenic research

authors： Buriani G,Mancini C,Benvenuto E,Baschieri S

更新日期：2011-04-01 00:00:00

abstract：:The MADS-box genes have been studied mainly in flower development by researching flower homeotic mutants. Most of the MADS-box genes isolated from plants are expressed exclusively in floral tissues, and some of their transcripts have been found in various vegetative tissues. The genes in the STMADS subfamily are impor...

journal_title：Transgenic research

authors： Shin MR,Seo SG,Kim JS,Joen SB,Kang SW,Lee GP,Kwon SY,Kim SH

更新日期：2011-04-01 00:00:00

abstract：:Multiple advantages-including the short generation time, large numbers of fertilized eggs, low cost of cultivation and easy maintenance favor the use of fish as bioreactors for the production of pharmaceutical proteins. In the present study, zebrafish eggs were used as bioreactors to produce mature tilapia insulin-lik...

journal_title：Transgenic research

authors： Hu SY,Liao CH,Lin YP,Li YH,Gong HY,Lin GH,Kawakami K,Yang TH,Wu JL

更新日期：2011-02-01 00:00:00

abstract：:Soybean was used as a model for studies of chemical induction of gene expression in seeds. A chimeric transcriptional activator, VGE, driven by the soybean seed glycinin G1 promoter, was used to induce the expression of an ER-targeted GFP(KDEL) reporter protein upon addition of the chemical ligand, methoxyfenozide. Th...

journal_title：Transgenic research

authors： Semenyuk EG,Schmidt MA,Beachy RN,Moravec T,Woodford-Thomas T

更新日期：2010-12-01 00:00:00

abstract：:Agrobacterium rhizogenes-transformed sugar beet hairy roots, expressing dsRNA from the Beet necrotic yellow vein virus replicase gene, were used as a novel approach to assess the efficacy of three intron-hairpin constructs at conferring resistance to rhizomania disease. Genetically engineered roots were similar in mor...

journal_title：Transgenic research

authors： Pavli OI,Panopoulos NJ,Goldbach R,Skaracis GN

更新日期：2010-10-01 00:00:00

abstract：:This study generated a transgenic zebrafish line Tg(k18:Ptmaa-RFP) with overexpression of Prothymosin alpha type a (Ptmaa) in the skin epidermis. Red fluorescence first appears very weakly in the early stage, become stronger and mainly restricted in the nuclei of the epithelial cells from 3 dpf-larvae to adult fish. H...

journal_title：Transgenic research

authors： Pai CW,Chen YH

更新日期：2010-08-01 00:00:00

abstract：:Supv3L1 is an evolutionarily conserved helicase that plays a critical role in the mitochondrial RNA surveillance and degradation machinery. Conditional ablation of Supv3L1 in adult mice leads to premature aging phenotypes including loss of muscle mass and adipose tissue and severe skin abnormalities. To get insights i...

journal_title：Transgenic research

authors： Paul E,Kielbasinski M,Sedivy JM,Murga-Zamalloa C,Khanna H,Klysik JE

更新日期：2010-08-01 00:00:00

abstract：:To construct an efficient system for the production of recombinant proteins in silkworm (Bombyx mori), we investigated the promoter activity of the silkworm sericin 1, 2, and 3 genes (Ser1, Ser2, and Ser3) using a GAL4/UAS binary gene expression system in transgenic silkworm. The promoter activity of the upstream regi...

journal_title：Transgenic research

authors： Tatematsu K,Kobayashi I,Uchino K,Sezutsu H,Iizuka T,Yonemura N,Tamura T

更新日期：2010-06-01 00:00:00

abstract：:Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce li...

journal_title：Transgenic research

authors： Kawazu Y,Fujiyama R,Noguchi Y,Kubota M,Ito H,Fukuoka H

更新日期：2010-04-01 00:00:00

abstract：:Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable transgenic BALB/c mice by perivitelline injection. In our vectors ...

journal_title：Transgenic research

authors： Kvell K,Czömpöly T,Hiripi L,Balogh P,Kóbor J,Bodrogi L,Pongrácz JE,Ritchie WA,Bosze Z

更新日期：2010-02-01 00:00:00

abstract：:We examined the justifications invoked by the German government in April 2009 to suspend the cultivation of the genetically modified maize varieties containing the Bt insect-resistance trait MON810. We have carried out a critical examination of the alleged new data on a potential environmental impact of these varietie...

journal_title：Transgenic research

authors： Ricroch A,Bergé JB,Kuntz M

更新日期：2010-02-01 00:00:00

abstract：:We generated a transgenic line Tg(k18:shh:RFP) with overexpression of Sonic hedgehog in the skin epidermis. By 5 day-post-fertilization (dpf), many epidermal lesions were clearly observed, including a swollen yolk sac, epidermis growth malformation around the eyes and at the basement of the pectoral fins. Skin histolo...

journal_title：Transgenic research

authors： Chen YH,Wang YH,Yu TH,Wu HJ,Pai CW

更新日期：2009-12-01 00:00:00